By Sharlene J. (Janice) Nagy Hesse-Biber, Patricia Lina Leavy
Feminist study perform: A Primer offers a special, hands-on method of exploring a number of feminist views of the study technique that allows you to bridge the divide among concept and examine equipment. Editors Sharlene Nagy Hesse-Biber and Patricia Lina Leavy have interaction scholars with a transparent and concise writing type and in-depth examples of a number of learn equipment from ethnography, oral heritage, concentration teams, and content material research to interviewing and survey study.
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GENOMICS OF NITROGEN FIXATION IN CLOSTRIDIA 17 4. ORGANIZATION OF THE NITROGEN-FIXATION GENE CLUSTER The complete or partial amino acid sequence of the C. , 1984) were determined with the purified protein. Therefore, the assignment of the nitrogenase structural genes, nifHDK, K in C. pasteurianum is definitive, although there is a lack of data from genetic tests. The other niff genes, which occur downstream to the nifHDK genes, are identified by the conserved amino-acid sequences they encode and by their positional relationships to the nifHDK K genes.
Tetani, although a member of Cluster I, is phylogenetically distant to C. perfringens and the diazotrophic Clostridium species. No nif-specific f genes have been identified by BLAST searches of the genomes of both C. perfringens and C. tetani; however, open-reading frames related to the non-niff genes, nirJ-1 and nirJ-2, which are present in the niff cluster of C. beijerinckii, can be found in the genomes of C. perfringens and C. tetani (Toth and Chen, unpublished results). The genomes of these non-nitrogen-fixing clostridia may be analyzed for structure, function, and regulation of genes involved: (i) in ammonia assimilation; (ii) in the electron-transport pathways toward the reduction of the low-potential electron carriers (ferredoxin and flavodoxin); and (iii) in the assimilation of iron, sulfur, and molybdenum, which are relevant to the nitrogenfixation process.
1. Free-living growth state Many of the phenotypic traits of N. N punctiforme enhance its photosynthetic competence and contribute to its competitive survival in a terrestrial habitat. N. , 2001). These properties are important in surface terrestrial habitats subject to both unshaded and changing shaded illumination. N. punctiforme can assimilate ammonium and nitrate/nitrite, in addition to dinitrogen (N2). Nitrogen fixation clearly provides a survival advantage in habitats lacking combined nitrogen.
Feminist Research Practice: A Primer by Sharlene J. (Janice) Nagy Hesse-Biber, Patricia Lina Leavy